Cytohistochemical Techniques
- Details
- Category: Discipline
- Bancroft JD, Stevens A. (2007). Theory and Practice of Histological Techniques. (6 th ed.).Edinburgh: Churchill Livingstone. Kiernan JA. (2015). Histological and Histochemical Methods. (5 th ed.). United Kingdom: Scion Publishing Ltd. Luna L. (1993). Histopathologic Methods and Color Atlas of Special Stains and Tissue Artifacts. American Histolabs Pub Dept. Carson FL, Hladik C. (2014). Histotechnology: A Self-Instructional Text. (4 th ed.). American Society for Clinical Pathology. Wick, MR. (2008). Diagnostic Histochemistry. (1 st ed.). New York: Cambridge University Press.
- Biomedical Laboratory Sciences
- 4084
- 19570
- Cytohistochemical Techniques
- IPLUSO4084-19570
- 3
- 5
- 0
- 4
- Não
- Português
- In practical classes, the student has prior access to the experimental protocols to be carried out in each class session, which allows for prior reading and preparation. These protocols are accompanied by a set of questions, to ensure individual preparation and, subsequently, the discussion of procedures and results.
- Mandatory
- Publicizing the main techniques of staining of cells and tissues and their applications in clinical pathology diagnosis and research. Identify the biochemical characteristics of a given cell or tissue component that allow the coloring / impregnation; Identify and describe the principles of coloring / impregnation of cell components and underlying tissues to a given histochemical method; Explain the principles of histochemical methods which reflect the different components and tissue molecules and cite the applications thereof in diagnostic Pathology; Identify the factors that influence the quality of different histochemical methods; Identify and describe the main methods for the evaluation of enzyme activity in a tissue; Identify and implement different types of controls used in histochemical and histoenzymatic methods; Observe and interpret the microscopic histochemical and histoenzymatic methods performed in practical classes and assess their quality.
- 1. Theory of Staining. Composition of white light and complementary colors. Batocromic effect and hypsochromic effect Natural dyes and synthetic dyes. Chemical structure and functional groups of the dyes. Classification of dyes. Auxochrome and chromophore. Mechanisms for linking the dye to tissue components. Factors influencing the differential staining of tissues. Direct and indirect staining. Progressive staining and regressive staining. Impregnation of heavy metals. Metachromasia. 2. Histochemistry Connective tissue. Carbohydrates Amyloid Lipids Pigments and precipitates Infectious agents Nucleic acid 3. Enzyme Histochemistry
Descrição Data limite Ponderação 1º Teste de avaliação teórico XX-XX-XXXX 25% 1º Teste de avaliação prático XX-XX-XXXX 25% 2º Teste de avaliação teórico XX-XX-XXXX 25% 2º Teste de avaliação prático XX-XX-XXXX 25% A classificação final (CF) da unidade curricular por avaliação contínua é obtida a partir das classificações obtidas na componente escrita (T) e na componente prática (PL). A componente escrita consta de duas frequências escritas e é relativa aos conteúdos teóricos. A classificação mínima é de 9,5 valores. A componente prática consta de 2 momentos de avaliação práticos realizados durante as aulas. A classificação mínima em cada frequência prática é de 9,5 valores. A classificação final é calculada a partir da seguinte fórmula: CF = 0,50 T + 0,50 PL. A classificação de todos os instrumentos de avaliação é expressa numa escala de 0 a 20 valores, sendo que o valor ponderado das classificações obtidas em cada componente, tem de ser igual ou superior a 9,5 valores.
- Semestral
- The physicochemical mechanisms of most histological stains are currently clarified. Histological staining methods, from acid dyes to metal impregnation, involve broadly similar physicochemical principles. The main theories on common staining procedures will be described and discussed, for the correct preparation and analysis of tissues in order to diagnose pathologies and cooperate in research activities, within the required quality parameters, as well as to facilitate the rational trouble-shooting, if problems are encountered.
