Immunohistochemistry and Molecular Pathology
- Details
- Category: Discipline
- Dabbs DJ (2019) Diagnostic Immunohistochemistry: Theranostic and Genomic Applications. (5 th ed.): Elsevier. Suvarna, S. K., Layton, C. L & Bancroft, J. D. (2019). Bancroft's theory and practice of histological techniques . (8th ed). Amersterdam: Elsevier Cook, D. J. & Warren, P. J. (2015). Cellular pathology: an introduction to techniques and applications . Banbury: Scion Publishing Ltd.
- Biomedical Laboratory Sciences
- 4084
- 19573
- Immunohistochemistry and Molecular Pathology
- IPLUSO4084-19573
- 3
- 5
- 0
- 4
- Não
- Português
- In practical classes, the student has prior access to the experimental protocols to be carried out in each class session, which allows for prior reading and preparation. These protocols are accompanied by a set of questions, to ensure individual preparation and, subsequently, the discussion of procedures and results.
- Mandatory
- Define and explain theoretical concepts associated with the practice of immunohistochemistry; Recognize the structure and function of an antibody and distinguish a polyclonal antibody to monoclonal antibody for their production, sensitivity, specificity and cross-reactivity; Describe and explain the theoretical underpinnings of the various immunohistochemical methods, including immunofluorescence and enzyme immunoassays; Identify and distinguish the different antigen retrieval methods routinely used and explain them; Name, identify and explain the various systems of revelation and various systems for signal amplification; Interpret and correctly evaluate the immunocytochemical markings; Analyze photos of preparations for immunocytochemical identification of artefacts associated with errors in the technical procedure; Identify possible causes for obtaining marking background and choose corrective measures for their elimination.
- 1.Immunohistochemistry and framework in routine practice in Pathology. 2.Basic concepts applied in immunocytochemistry: antigens; Antibodies, antibody structure and subtypes; and antigen-antibody binding. 2.1. Immunohistochemical methods 2.1.1.Direct method 2.1.2.Indirect methods 2.2. Antigenic recovery 2.2.1.Enzyme recovery 2.2.2.Heat-induced recovery 2.2.2.1.Recovery Solutions 3.Blocking of endogenous substances 4.Automation in Immunohistochemistry 5.Quality control in Immunohistochemistry 5.1.Internal Quality Control 5.2.External Quality Control 6.Diagnosis in immunocytochemistry - Tumor markers in diagnostic routine in Pathology 7.Molecular Pathology 7.1.Techniques in Diagnostic Molecular Pathology 7.1.1.Key molecules used 7.1.2.Types of molecules 7.1.3.Importance of molecules used
Descrição
Data limite
Ponderação
Teste de avaliação teórico I
dd-mm-yyyy
25%
Teste de avaliação teórico II dd-mm-yyyy 25% Teste de avaliação prática I
dd-mm-yyyy
25%
Teste de avaliação prática II
dd-mm-yyyy
25%
A classificação final (CF) da unidade curricular por avaliação contínua é obtida a partir das classificações obtidas na componente escrita (T) e na componente prática (PL). A componente escrita consta de duas frequências escritas e é relativa aos conteúdos teóricos. A classificação mínima é de 9,5 valores. A componente prática consta de 2 momentos de avaliação práticos realizados durante as aulas. A classificação mínima em cada frequência prática é de 9,5 valores. A classificação final é calculada a partir da seguinte fórmula: CF = 0,50 T + 0,50 PL. A classificação de todos os instrumentos de avaliação é expressa numa escala de 0 a 20 valores, sendo que o valor ponderado das classificações obtidas em cada componente, tem de ser igual ou superior a 9,5 valores.
- Semestral
- This unit allows students to understand the basis of molecular pathology, the main techniques and molecules used in the diagnosis and its importance in the context of pathology.
